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詳細介紹
大鼠髓系細胞觸發(fā)受體-1 ELISA試劑盒
英文名稱：Rat Triggering Receptor Expresses on Myeloid Cells-1,TREM-1 ELISA KIT
Assay procedure
1. Dilute and add sample:Dilute Original density Standard as follow table:
| 8 pg/ml | 5 Standard | 150μl Original density Standard+150μl Standard diluent |
| 4pg/ml | 4 Standard | 150μl 5 Standard+150μl Standard diluent |
| 2 pg/ml | 3 Standard | 150μl 4 Standard+150μl Standard diluent |
| 1 pg/ml | 2 Standard | 150μl 3 Standard +150μl Standard diluent |
| 0.5 pg/ml | 1 Standard | 150μl 2 Standard +150μl Standard diluent |
2.add sample：Set blank wells separay （blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same）. testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl （sample final dilution is 5-fold）, add sample to wells , don’t touch the well wall as far as possible, and Gently mix.
3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37℃.
4.Configurate liquid: 30-fold wash solution diluted 30-fold （or 20-fold） with distilled water and reserve.
5.washing：Uncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.
6.add enzyme：Add HRP-Conjugate reagent 50μl to each well, except blank well.
7.incubate：Operation with 3.
8.washing：Operation with 5.
9.color：Add Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37℃
10.Stop the reaction：Add Stop Solution50μl to each well, Stop the reaction（the blue color change to yellow color）.
11.assay：take blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.
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