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GE 用于基團特異性純化親和預裝柱
GE 用于基團特異性純化親和預裝柱
貨號 | 預裝柱 | 平均 顆粒 (μm) | 每柱載量 | 應用/特性
| zui高流速(ml/min ) | 耐反壓(MPa) | pH 穩定性工作 | ||||
用于基團特異性純化 | |||||||||||
17-0412-01 | HiTrap Blue HP, 1 ml × 5 | 34 | > 18 mg HSA | 純化抗凝血激酶、核酸酶、NAD + / NADP+ 依整 酶、ATP 依賴酶、脂蛋白、干擾素、白蛋白等等 | 4 | 0.3 | 4-12 | ||||
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17-0413-01 | HiTrap Blue HP, 5 ml × 1 | 34 | > 175 mg HSA | 同上 | 20 | 0.3 | 4-12 | ||||
17-0406-01 | HiTrap Heparin HP, 1 ml × 5 | 34 | 3 mg | 純化抗凝血激酶和別的凝集因子，脂蛋白、 | 4 | 0.3 | 5-10 | ||||
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| antithrombin lll | 脂酶、蛋白合成因子、激素、類固醇受體、 |
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| DNA 結合蛋白、干擾素 |
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17-0407-01 | HiTrap Heparin HP, 5 ml × 1 | 34 |
| 同上 | 20 | 0.3 | 5-10 | ||||
17-0407-03 | HiTrap Heparin HP, 5 ml × 5 |
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17-5189-01 | HiPrep 16/10 HEPARIN FF | 90 | 2 mg antithrombin III | 同上 | 10 | 0.15 | 4-12 | ||||
17-5112-01 | HiTrap Streptavidin HP, 1 ml × 5 | 34 | 6 mg 生物素化的BSA | 利用生物素和抗生素的結合作用做親和層 | 4 | 0.3 | 2-10 | ||||
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| 析;如使用生物素標記的抗體純化相應抗原 |
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28-9031-30 | Streptavidin HP Spin Trap 16 × 100 ml | 34 | 6 mg 生物素化的BSA | 快速、方便，小量純化生物素化的基質，如 |
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28-9135-68 | Streptavidin HP Spin Trap Buffer kit |
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| 生物素標簽蛋白 |
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28-9031-31 | Streptavidin HP MultiTrap | 34 | 6 mg 生物素化的BSA | 快速、方便，高通量純化生物素化的基質，如 |
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| 4 × 96well filter plates |
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| 生物素標簽蛋白 |
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17-5143-02 | HiTrap BenzamidineFF, 2 × 1 ml | 90 | 35 mg 胰蛋白酶原 | 專門純化絲氨酸蛋白酶，胰蛋白酶和類胰 | 4 | 0.3 | 1-9 | ||||
17-5143-01 | HiTrap BenzamidineFF, 5 × 1 ml | 90 |
| 蛋白酶 | 4 | 0.3 | 1-9 | ||||
17-5144-01 | HiTrap BenzamidineFF, 1 × 5 ml | 90 | 175 mg 胰蛋白酶原 | 同上 |
| 0.3 | 1-9 | ||||
十四］用于金屬離子螯合純化 |
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17-0920-03 | HiTrap IMAC HP, 1 ml × 5 | 34 | 15 umol Ni2+/Zn2+/ml 膠 | 可與金屬作用的蛋白、肽類、核苷酸 | 4 | 0.3 | 3-12 | ||||
17-0920-05 | HiTrap IMAC HP, 5 ml × 5 |
| 25 umol Cu2+/ml 膠 |
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| [2-14] | ||||
17-0921-02 | HiTrap IMAC FF, 1 ml × 5 | 90 | 15 umol Ni2+/Zn2+/ml 膠 | 可與金屬作用的蛋白、肽類、核苷酸 | 20 | 0.3 | 3-12 | ||||
17-0921-04 | HiTrap IMAC FF, 5 ml × 5 |
| 25 umol Cu2+/ml 膠 |
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| [2-14] | ||||
17-0921-06 | HiPrep IMAC 16/10 20 ml × 1 |
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17-0408-01 | HiTrap Chelating HP, 1 ml × 5 | 34 | 23 umol Cu2+/ml 膠 | 純化含暴露氨基酸的蛋白／肽：His (Cys,Trp）， | 4 | 0.3 | 3-13 | ||||
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| 如巨球蛋白、干擾素 |
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17-0409-01 | HiTrap Chelating HP, 5 ml × 1 | 34 | 23 umol Cu2+/ml 膠 | 同上 | 20 | 0.3 | 3-13 | ||||
17-0409-03 | HiTrap Chelating HP, 5 ml × 5 |
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17-0409-05 | HiTrap Chelating HP, 5 ml × 100 |
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28-9298-81 | Phos Spin Trap Fe | 90 |
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| 離心 |
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| 2.5 ml media and 24 spin column |
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