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產品英文名稱：BHK HCP ELISA kit
產品中文名稱：BHK 細胞 ELISA 試劑盒
BHK HCP ELISA kit 的應用
本試劑盒是用于確定在BHK細胞中表達的制成品的宿主細胞蛋白污染的存在。該試劑盒是用于研究和制造使用，不用于人類或動物的診斷應用。
BHK 細胞 ELISA 試劑盒的英文描述
The BHK (Baby Hamster Kidney Fibroblast) cell line is used as an expression system for viral vaccines and other therapeutic proteins. Our HCP kit is generic in the sense that it is intended to react with essentially all of the HCPs that could contaminate the product independent of the purification process. The antibodies have been generated against and affinity purified using a mild lysate of BHK cells. The resulting antibodies have then been characterized against 3 commercial cell lines used to produce various viral and protein products. This analysis indicated the vast majority of HCPs were conserved among multiple BHK cell lines and product purification processes. Our assay validation has determined that the LOD for this kit is ~0.4ng/mL.
BHK HCP ELISA ki t的使用步驟規則
The BHK cell assay is a two-site immunoenzymetric assay. Samples containing BHK HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-BHK cell antibody (goat polyclonal) in microtiter strips coated with an
affinity purified capture goat polyclonal anti-BHK cell antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of BHK cell HCPs present.
BHK HCP ELISA kit 的保存和穩定性
* Store Standards at –10°C to -30°C. All other reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.
* Reconstituted wash solution is stable until the expiration date of the kit.
BHK HCP ELISA kit 的使用注意事項
*只用于研究或制造。
*停止反應試劑是0.5N H2SO4。避免接觸眼睛，皮膚，和服裝。在用本試劑盒的試劑濃度，其他沒有被認為是有害的。
*本試劑盒只能由合格的技術人員使用。
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