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Gibco/12100046/高糖DMEM干粉/現(xiàn)貨
閱讀：2169 發(fā)布時(shí)間：2017-12-29| 12100046 | DMEM, powder, high glucose |
Gibco/12100046/高糖DMEM干粉/現(xiàn)貨
訂貨找螞蟻淘就對(duì)了，4000-520-626
描述
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Phenol Red | • Sodium Bicarbonate |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For In Vitro Diagnostic Use.
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螞蟻淘常見平行進(jìn)口產(chǎn)品有：
Thermofisher旗下Gibco培養(yǎng)系列、ABI細(xì)胞計(jì)數(shù)儀及PCR儀、Invitrogen分子生物學(xué)試劑及Qubit3.0、Ambion的RNA相關(guān)試劑、Molecular probes試劑、Pierce蛋白相關(guān)試劑盒、Nanodrop One/OneC；Parafilm封口膜；Viskase透析袋；Sigma-Aldrich試劑；AccuStandard 標(biāo)準(zhǔn)品；Millipore抗體；Glen Research 核酸合成試劑；Bio-Rad電泳儀及PCR儀；Nanocs的PEG聚合物； Chromotek羊駝抗體； Polysciences轉(zhuǎn)染試劑； Echelon-inc 脂類研究試劑盒； R&D Systems抗體及Elisa試劑盒； Jackson二抗及封閉血清； Biotium 熒光染料；NEB內(nèi)切酶及NGS相關(guān)產(chǎn)品； Athens Research & Technology人類蛋白及抗血清； Illumina二代測(cè)試試劑盒； Tocris 小分子試劑； TwistDx恒溫?cái)U(kuò)增試劑盒（RPA）； Enzymatics二代測(cè)序酶；Novus抗體及Elisa試劑盒；AG scientific潮霉素B、G-418；Plantmedia植物培養(yǎng)系列及抗體；Agilent 2100生物分析儀；Biolog耗材；Biomatik的Elisa試劑盒；Covaris基因打斷儀；CST抗體；Dharmacon的siRNA產(chǎn)品。